Connecting signaling and metabolic pathways in EGF receptor-mediated oncogenesis of glioblastoma
Fig 9
Test of the GBM-specific significant network model in cell cultures.
The EGFRwt and EGFRvIII-mutated GBM cell lines U87MG and U87MGvIII were incubated with inhibitors for calmodulin (CALM2) with CGS 9343B (A), casein kinase II subunit alpha (CSNK2A1) with Emodin (B), 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 (PLCG1) with U73122 (C), tyrosine-protein kinase ABL1 with Dasatinib(D), and B-cell lymphoma 2 (BCL2) with ABT-199 (E). The bar diagrams on the left show the effects of the inhibition of the expression of metabolic pathway proteins predicted to be interconnected to the targeted signaling pathway proteins. The line graphs on the right show the dose-dependent effects of the signaling protein inhibitors on the viability of the cells.