Neural crest streaming as an emergent property of tissue interactions during morphogenesis
Fig 6
Emergent streams at different neural crest adhesion strengths.
(A, B, E) Final cell configurations in simulations with normal (A), low (B), and high (E) neural crest adhesion parameters (λ_M (NC-NC)). (C, D, F) Streams revealed by ISH in control (C), LPAR2-overexpressing (D), and LPAR2-Mo injected (F) embryos. (G) Stream widths in vivo measured in control embryos and embryos injected with either LPAR2 mRNA (OE, overexpression) or LPAR2-Mo (Mo). Error bars: SEM; n(Ctrl, MO) = 30; n(OE) = 19; ***: p<0.001 Mann-Whitney test. (H) Stream width and length in silico as a function of λ_M (NC-NC). Error bars: SEM. (I, J) Morphological maps of simulated NC streams at various adhesion-placodal CIL, and adhesion-chemotaxis parameters. Box width and length corresponds to width and length of streams. Box shading from light to dark corresponds decreasing relative standard error of width and length measures. Letters refer to related panels. N = 20 simulations for each parameter set. Scalebars: 300μm.