Neural crest streaming as an emergent property of tissue interactions during morphogenesis
Fig 5
Attraction at a distance and repulsion at a shorter range is required for neural crest stream formation.
(A-B) Control (A) and inhibition of Sdf1 chemotaxis (B) in simulations. Representative cell configurations at the end of simulations (t = 3600MCS). (C-D) ISH of post-migratory stage embryo injected with control-Mo (CoMo, C) showing normal NC migration and CxcR4-Mo (D) showing lack of NC migration. (E) Width and length of streams measured from the density probability functions of simulations with different chemotaxis parameters. Arrow indicates default value. (F) Inhibition of CIL in placodal cells in simulations. Configurations of cells at the end of simulation (t = 3600MCS). (G) NC shown by ISH in wild-type embryos with placodes grafted from DshDEP+ injected embryos. (H) Width and length of simulated streams as in (E), corresponding to (F) and (A). Arrow indicates default value. (I) Morphological map of simulated NC streams at various chemotaxis and placodal CIL parameters. Box width and length corresponds to width and length of streams. Box shading from light to dark corresponds decreasing relative standard error of width and length measures. Letters refer to related panels. Error bars on E and H: SEM. N = 20 simulations for each point on E, H, and I. Scalebars: 200μm.