Model-based analysis of influenza A virus replication in genetically engineered cell lines elucidates the impact of host cell factors on key kinetic parameters of virus growth
Fig 1
Virus release dynamics in response to in silico manipulation of gene expression of host cell factors in A549 cells.
We assume that the efficiency of individual steps in the virus life cycle is directly dependent on host cell factors and their influence is changed upon knockdown or overexpression of the corresponding gene. We simulated manipulation of gene expression by perturbing the corresponding kinetic parameters of an IAV replication model for A549 cells, which is based on a model previously established by our group [12]. For a simulated infection at MOI 1, virus release of the parental A549 (blue solid line) and the engineered cell line (brown solid line) are shown for the most important virus replication steps. Colors indicate whether perturbation of the corresponding step improved virus yield at 24 h p.i. by at least two-fold (green), had only an impact on the starting time point of virus release (yellow) or no impact (red). Scheme of IAV replication adapted from [22].