Predicting proteome allocation, overflow metabolism, and metal requirements in a model acetogen
Fig 6
Effects of nickel availability on C. ljungdahlii grown on fructose.
(A) Predicted growth rate and protein activity of carbon monoxide dehydrogenase:acetyl-CoA synthase (CODH_ACS) were plotted against relative nickel uptake (mmol*gDW-1*h-1). (B) Predicted ethanol (EtOH) secretion at optimal nickel uptake (WT) and no available nickel (-Ni2+) were plotted against relative fructose uptake (mmol*gDW-1*h-1). (C) Predicted acetate (Ac) secretion at optimal nickel uptake and no available nickel were plotted against relative fructose uptake (mmol*gDW-1*h-1). Measured (D) growth rate, (E) fructose consumption, (F) final ethanol concentration, and (G) final acetate concentration of fructose-grown C. ljungdahlii without added nickel and with ten times the concentration of nickel were plotted (±std, n = 3). Gray asterisk indicates difference significance is p = 0.06, and three black asterisk indicates significance of p<0.001.