Investigating the deregulation of metabolic tasks via Minimum Network Enrichment Analysis (MiNEA) as applied to nonalcoholic fatty liver disease using mouse and human omics data
Fig 4
Minimal network enrichment analysis (MiNEA) overview.
For each metabolic task (MT), MiNEA computationally enumerates MiNs that comprise active reactions for the MT. The inputs required for the MiNEA analysis are a genome-scale metabolic network (GEM), a given list of metabolic tasks (MTs), and transcriptomics data. Using this, alternative minimal networks (MiNs) are enumerated for MTs using a GEM. (steps 1–3). Transcriptomics data are used to identify differentially regulated genes between two conditions (step 4). To identify deregulated MTs and their associated MiNs, a hypergeometric test is performed with a set of deregulated genes (step 5).