Modeling cell line-specific recruitment of signaling proteins to the insulin-like growth factor 1 receptor
Fig 4
Predicted recruitment of signaling proteins to IGF1R in HeLa S3 cells.
The scale represents the normalized amount of each of the indicated proteins bound in simulations of either (A) time-dependent recruitment at 1 nM IGF1 stimulation, or (B) steady-state recruitment at varying IGF1 doses. (C) Comparison of rank ordering of IGF1R binding partners in HeLa S3 cells across five prediction methods. The top (first) ranked protein indicates the protein predicted to be bound to IGF1R with the highest abundance. Bar length indicates the amount of deviation from the ranking obtained by numerical simulations for estimates obtained by the analytical approach, copy number, by KD, or the ratio of copy number to KD. A negative deviation indicates that the method estimated a lower rank (less binding) than the prediction from numerical simulations; a positive deviation indicates a higher rank (more binding) than in numerical simulations. Calculation and evaluation of rank ordering are described in Materials and Methods.