Modeling cell line-specific recruitment of signaling proteins to the insulin-like growth factor 1 receptor
Fig 3
Parameter values for IGF1-IGF1R binding were obtained by fitting to data from competition experiments and ligand dissociation experiments presented by Kiselyov et al. [44]. Experimental data are shown as points with error bars and the smooth curves were obtained via numerical simulation. For (A), we simulated to steady-state for a range of concentrations of unlabeled IGF1. For (B), we simulated a two-hour incubation with radiolabeled IGF1, then free ligand was washed away (i.e., the abundance of free IGF1 was set to zero), and we simulated the response to ligand addition for either 20 or 60 minutes, as indicated, for a range of concentrations of unlabeled IGF1.