CoPhosK: A method for comprehensive kinase substrate annotation using co-phosphorylation analysis
Fig 5
The correspondence between the predictions of CophosK vs. KinomeXplorer in ranking kinases for each phosphosite.
For all kinase-substrate associations reported in PhosphoSitePlus for which we can detect a substrate in the LC/MS data, we perform leave-one-out cross validation by hiding the association between the phosphosite and kinase and using CophosK to utilize other kinase-substrate associations and co-phosphorylation to rank the likely kinases for the phosphosite. (a) shows the comparison of the rankings provided by CoPhosK (x-axis) against the rankings provided by KinomeXplorer (y-axis) for breast cancer PDX data (740 predictions) and ovarian cancer (313 predictions). In (b), the box plot distribution of the rank of the target kinase according to the prediction of two methods are presented.