CoPhosK: A method for comprehensive kinase substrate annotation using co-phosphorylation analysis
Fig 3
Distribution of co-phosphorylation between phosphosites that are substrates of the same kinase.
(a) For each of 347 reported kinases, we compute the co-phosphorylation of all pairs of phosphosites that are reported to be common substrates of that kinase in PhosphositePLUS (shared-kinase pairs). In panels (b), (c), the green histogram shows the distribution of co-phosporylation for all shared-kinase pairs and the blue histogram shows the distribution of co-phosphorylation for all pairs of phosphosites in the dataset. (b) Breast Cancer PDX dataset (37234 shared kinase pairs; μ = 0.05,σ = 0.23,kutosis = 2.85, skewness = 0.10), (c) Ovarian Cancer tumors (8235 shared kinase pairs; μ = 0.11,σ = 0.32,kutosis = 2.54, skewness = -0.10). For both datasets, the distribution for shared-kinase pairs is significantly wider and shifted to the right as compared to the distribution for all phosphosite pairs (KS-test p-value << 1E-9).