Chemogenomic model identifies synergistic drug combinations robust to the pathogen microenvironment
Fig 1
Antibiotic interactions change significantly in different growth environments.
(a) Heat maps represent all pairwise interactions among 8 antibiotics in rich media (LB) and minimal media supplemented with glucose. Blue, white or red boxes correspond to synergistic, additive or antagonistic pairs. All drug combinations on average showed a significant shift towards synergy in glucose media. This was strongly pronounced for combinations involving both bactericidal and bacteriostatic drugs. The average interaction score for each class of drug combinations is shown in the table. (b) Scatter plot comparison of interaction scores in rich media versus glucose minimal media. The log transformed FIC interaction score is shown. While there is a significant correlation between interaction scores in the different media conditions, there are salient differences. Outlier combinations that have divergent outcomes in each condition are highlighted. For example, ampicillin is synergistic with azithromycin only in minimal media.