Review: Precision medicine and driver mutations: Computational methods, functional assays and conformational principles for interpreting cancer drivers
Fig 9
(A) Akt1 is composed of PH (residues 5–108), kinase domain (residues 150–408), and regulatory domain (residues 409–480). The Gly-rich α-helix linker connects the PH and kinase domains. (B) Non-hotspot, passenger mutations can be functional as the driver mutation E17K. The PH domain mutants, L52R, C77F, and Q79K activate Akt1 as the E17K driver does, whereas D32Y, K39N, and P42T mutants at the interface between the PH and kinase domains reduce Akt1 in the inactive conformation. PH, pleckstrin homology.