Molecular basis for the increased affinity of an RNA recognition motif with re-engineered specificity: A molecular dynamics and enhanced sampling simulations study
Fig 1
(A) NMR structure of pre-miR20b RNA (pdb 2n7x). (B) NMR structure of the Rbfox•pre-miR20b complex (pdb 2n82). The residues at the binding interface are highlighted. The amino acids labelled with a red square correspond to the mutated residues in Rbfox* and in Rbfox* S151T. (C) Left: nucleotide sequence of the pre-miR20b and of the mutant pre-miR20b*. Right: amino acids sequence of the Rbfox, Rbfox* and Rbfox* S151T mutants. Highlighted in green and in orange are the amino acids corresponding to β strands and α helices, respectively. The mutated nucleotides and amino acids are underlined.