Coevolving residues inform protein dynamics profiles and disease susceptibility of nSNVs
Fig 9
Flowchart of Seq-GNM method for nSNV predictions.
A workflow of our method to use predicted evolutionary couplings to determine protein dynamics and assess the functional impact of nSNVs. The initial input is an amino acid sequence, which is used to obtain MSA. Using MSA evolutionary coupling pairs are predicted through RaptorX and EVcouplings. The high scored evolutionary coupling pairs are assigned as contacts in our Seq-GNM to compute the dynamics profile of each protein. The dynamic profiles obtained from Seq-GNM can give insight into the functional impact of nSNVs. This was done for a curated set of 139 structures.