Rotation of sex combs in Drosophila melanogaster requires precise and coordinated spatio-temporal dynamics from forces generated by epithelial cells
Fig 9
Experiments showing rotation of longer SCs exhibits delayed expansion of distal epithelial cells closer to the base of comb.
A Left: confocal image of short SC and its surrounding cells at the start of area measurement (23 hours AP). Shaded areas (magenta and blue) are the cells selected for area measurement. Middle: same confocal image with identical shaded cells at 36 hours AP (end of area measurement at 41.5 hours AP). Right: graph of percentage change of apical areas of the shaded cells vs. time and the fitted logistic curves. Each coloured straight line is the tangent at the inflexion point of the same coloured logistic curve. Its intercept with the time axis is mathematically constructed as the start of the expansion phase of the cells. Each scale bar: 20 μm. B Same as A but for wildtype comb. Start of area measurement at 22 hours AP. C Same as B but for longer comb. Start of area measurement at 22 hours AP.