Profiling cellular morphodynamics by spatiotemporal spectrum decomposition
Fig 7
Regions of spectrally homogeneous cell edge motion have distinct Rac1 signaling programs.
(a) FRET biosensor probing Rac1 activity (yellow-red tones, high activity; green-blue tones, low activity) in a Cos7 cell. The rectangular boxes with white edges overlaid on cell periphery represent the first (outer) and second (inner) layer of sampling windows. The numbers along the cell periphery mark the locations of sectors/windows. Scale bar = 30 μm. (b) Rac1 activity map of a Cos7 cell. (c) Protrusion activity map of a Cos7 cell. (d) SRM identifying four distinct cell edge motion regimens. (e) Quantification of Rac1 activity in the first layer of probing windows for spectral clustering regimens 1–4. (f) Spectral clustering regions in the first layer of probing windows at six time points of the time lapse sequence (see also Video 9 for the full sequence). Scale bar = 30μm. (g-j) Cross-correlation between edge motion and Rac1 activity in the first and second layer of probing windows for regimens 1–4.