Profiling cellular morphodynamics by spatiotemporal spectrum decomposition
Fig 3
Acute photo-inhibition (PI) of Vav2 signaling induces a shift in the instantaneous frequency distribution.
(a) Timing of Rac-GEF Vav2 photo-inhibition using PI Vav2. (b) Comparison of IMF1 instantaneous frequency distributions collected before and during a PI period composed of 1000 msec pulses of light interspersed with 9000 msec darkness, 100 msec pulses of light interspersed with 9900 msec darkness, and 1 msec pulses of light interspersed with 9999 msec darkness (from top to bottom). P-value is calculated by K-S test. Data for IMF2-6 are presented in S4 Fig. (c) Representative scatter plots of instantaneous frequency vs amplitude values of IMF1 before and during a PI period induced by 1000, 100 and 1 msec of light pulses (from top to bottom), respectively.