Predicting 3D structure and stability of RNA pseudoknots in monovalent and divalent ion solutions
Fig 6
The comparisons between predictions (lines) and experiments (symbols) for MMTV and T2 pseudoknots in divalent ion solutions.
(a) MMTV pseudoknot at 50mM [K+] with 0.5mM [Mg2+] and 3.0mM [Mg2+], respectively. Symbols: the heat capacity Cp for MMTV pseudoknot at 50mM [K+] with 0.5mM [Mg2+]. (c) T2 pseudoknot at 100mM [K+] with 0.1mM [Mg2+] and 1.0mM [Mg2+], respectively. Symbols: dA/dT, the first derivative of absorbance with respect to temperature. (b, d) The melting temperatures Tm1 and Tm2 of two transitions (F→I and I→U) as functions of [Mg2+] for MMTV pseudoknot in the presence of 50mM [K+] (b) and T2 pseudoknot in the presence of 100mM [K+] (d). Symbols: experimental Tm1 (blue square) and Tm2 (red triangle) [75,77]. Lines: corresponding predictions.