Predicting 3D structure and stability of RNA pseudoknots in monovalent and divalent ion solutions
Fig 5
The comparisons between predictions (lines) and experiments (symbols) for MMTV and T2 pseudoknots in monovalent ion solutions.
(a) MMTV pseudoknot at 1000mM [K+] (green square) and 50mM [K+] (red triangle), respectively. Lines: df/dT, the first derivative of f with respect to temperature. Symbols: the heat capacity Cp. (c) T2 pseudoknot at 1000mM [K+] (green square) and 100mM [K+] (red triangle), respectively. Symbols: dA/dT, the first derivative of absorbance with respect to temperature. (b, d) The melting temperatures Tm1 and Tm2 of two transitions (F→I and I→U) as functions of [K+] for MMTV (b) and T2 (d) pseudoknots. Symbols: experimental Tm1 (blue square) and Tm2 (red triangle) [75,77]. Lines: corresponding predictions.