Control of neurite growth and guidance by an inhibitory cell-body signal
Fig 2
Analysis of NGF gradient data set.
(A) Example image from the data set of ref. [18], which documents the total growth of DRG explants after 48 h in shallow NGF gradients. Experimental conditions were varied over gradient steepnesses 0 − 0.3% concentration change per 10 μm, and background concentrations of ≈ 0.001 − 100 nM (scale bar 500 μm). (B) Average radial outgrowth, quantified by Fourier coefficient a0, has a biphasic dependence on background NGF concentration, and is largely independent of gradient steepness. (C) Directional bias up the gradient, quantified by normalised coefficient b1/a0, was observed over background concentrations 0.01 − 1 nM, implying a high sensitivity to NGF.