miRAW: A deep learning-based approach to predict microRNA targets by analyzing whole microRNA transcripts
Fig 7
Example of a miRNA binding site that can accommodate a miRNA (hsa-miR-21) with different binding patterns and different site stabilities.
The left figure shows a canonical binding (perfect 7mer) with ΔGduplex = −10.30kcal/mol while the right figure shows a non-canonical binding (containing wobbles in the seed region) ΔGduplex = −11.70kcal/mol. While the left structure can be identified by both canonical and non-canonical CSSMs, a non-canonical CSSM will preferentially select the right hand structure as a potential MBS since it reports a more stable predicted binding energy.