Parameter uncertainty quantification using surrogate models applied to a spatial model of yeast mating polarization
Fig 6
MCMC vs. optimization results.
Steady state solutions for the mean parameter set from the MCMC (solid black) and a parameter set identified via simulated annealing (dashed black). Polarization is depicted by the concentration of active Cdc42 (C42a, number of molecules/μm2) over the angular range [−π, π]. The mean polarization of the experimental data (n = 20 cells) is shown in blue in arbitrary units. A sample cell, treated with 10 nM α-factor for 60 min, shows the membrane polarization profile of Ste20-GFP, a reporter for active Cdc42 (upper left). Scale bar = 2 μm.