Organization of the core respiratory network: Insights from optogenetic and modeling studies
Fig 14
Model-data comparisons for responses to stimulations of inhibitory and excitatory pre-BötC populations.
(A) Integrated recording of diaphragmatic EMG (ʃDIAEMG) activity and laser activation illustrating that continuous ChR2 photostimulation of excitatory and inhibitory pre-BötC together induces an increase in respiratory frequency (data from the study of Alsahafi et al. [47], Fig 2B, used with authors’ permission). The lower five traces show the activity of all neuron populations in the model. The applied photostimulations are shown at the bottom (stimChR = 0.18). The timing of the laser stimulus is indicated with blue shading and dashed red lines. Stimulating all populations in the model elicited a frequency increase similar to the experimental data. (B) Recording of ʃDIAEMG and laser frequency during 1 Hz photostimulation showing entrainment of the respiratory rhythm to the periodic stimulation (data from the study of Alsahafi et al. [47], Fig 6B, used with authors’ permission). The lower five traces show the activity of all neuron populations in the model. The applied stimulations are shown at the bottom (stimChR = 0.2), and the timing of the laser stimulus is indicated with blue shading and dashed red lines. Stimulating all pre-BötC populations in the model can entrain the network activity at the experimental photostimulation frequency of 0.67Hz.