Mechanical unfolding reveals stable 3-helix intermediates in talin and α-catenin
Fig 5
Unfolding patterns of tandem α-helical bundles as determined by smAFM.
(a-d) Talin R9 (wt)–R9 (wt) (47 traces). (e-h) R9 (N/C-clamps)–R9 (wt) (34 traces). (i-l) α-catenin (MI-MII) (40 traces). (a, e & i) length histogram indicating the distance from unfolding event to HaloTag standard; (b, f & j) scatter plot showing force vs position for all unfolding events; histogram the force associated with unfolding events; (d,h & l) representative force extension retraction curve (blue, cyan & black line) showing how the unfolding events relate to scatter plot and length histogram (black dotted line), gray line represents approach curve for AFM tip. Gray dotted lines on (c, g & k) histograms represent Gaussian fits.