RosettaAntibodyDesign (RAbD): A general framework for computational antibody design
Fig 8
Designed antibodies against bee hyaluronidase.
(A) Apparent Binding Affinity (KD) of expressed and tested antibody designs for bee hyaluronidase (PDB: 2J88), grouped by designed CDR cluster, as determined using Surface Plasmon Resonance (SPR) on a Biacore 4000. The dotted blue line represents the binding affinity of the native antibody on the Biacore machine (1.57x10-8 nM). Binding affinity is shown for the 26 designs that had detectable binding affinity (out of 30 tested). The native CDRs are L1-11-2 and H2-9-1. (B) Kinetic sensorgrams of WT 2J88 Antibody to Bee Hyaluronidase. Two repeats of XPR (left); Biacore 4000 (right). (C) Kinetic sensorgrams of design L14_7 to Bee Hyaluronidase Two repeats of XPR (left); Biacore 4000 (right). (D) Model of the interface changes in design L14-7, with designed L1 cluster L1-11-2 (cyan), superimposed onto the WT antibody from PDB ID 2J88 (gray). (E) Designed Sequences vs WT.