Using pseudoalignment and base quality to accurately quantify microbial community composition
Fig 3
151bp and 301bp simulation results.
Average relative errror (AVGRE) with 95% confidence intervals from the taxonomic quantification of simulated samples with 151bp paired-end and 301bp paired-end reads. Per reference error was calculated using the classifications from Karp, Kallisto, UCLUST, USEARCH, and SortMeRNA Only references with frequencies >0.1% in either the true reference or a simulated sample were used to calculate error. (A) Per reference error in full 16S gene samples of 151bp paired-end reads. (B) Per reference level error in 151bp paired-end samples simulated using only the V4 hypervariable region of the 16S gene. (C) Per reference error in full 16S gene samples of 301bp paired-end reads. (D) Per reference error in V4 samples of 301bp paired-end reads.