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New methods for computational decomposition of whole-mount in situ images enable effective curation of a large, highly redundant collection of Xenopus images

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Validation of image classification and embryo detection algorithms: Identification of non-subjective errors through visual inspection of 2000 randomly selected images.

Image classification: was the image correctly classified as cleared or un-cleared? Background segmentation: was the background component identified correctly? Embryo region selection: did the connected region selected correspond to the embryo? Incomplete embryo detection: were embryos touching the image edge correctly identified as such?

Table 1

doi: https://doi.org/10.1371/journal.pcbi.1006077.t001