New methods for computational decomposition of whole-mount in situ images enable effective curation of a large, highly redundant collection of Xenopus images
Fig 4
Simplified example of spherical stage image similarity clustering.
Early development stages are routinely photographed from different directions to maximise information about the expression pattern. Reference and comparison images for the same gene and stage are compared under multiple transformations (scale, rotation, shear) to identify the set of transformations that minimises their dissimilarity. Here we see that a 91.5o rotation and a 1.01 scaling suggest the most likely transformation between these two images. All images from the same gene and stage are compared with each other to identify images from (probably) the same view-point. See also Fig 3B.