An open source tool for automatic spatiotemporal assessment of calcium transients and local ‘signal-close-to-noise’ activity in calcium imaging data
Fig 9
Structures with high rates of local activity after calcium spike blockade.
a, Average intensity image of hippocampal neurons loaded with the calcium indicator. Grid windows with high rates of local activity are shown in yellow (see c). b, Activity map (see also Fig 8). c, Five regions of interest are indicated (trace 1 –trace 5). The upper three represent growth cone-like structures, the lower two traces represent hotspots on neurites. Note the variability and the diverse character of the calcium signal patterns detected by the computational approach.