An open source tool for automatic spatiotemporal assessment of calcium transients and local ‘signal-close-to-noise’ activity in calcium imaging data
Fig 7
Activity profile of hippocampal neurons after calcium spike blockade.
a, Average intensity: Hippocampal neurons loaded with a fluorescent calcium indicator. Pseudocolor images: neurons before a spike, during a calcium spike (seen in the lower left cell), and after spike blockade. 3000 images, acquired at 20Hz are analyzed (WS8, SAT7, SNR 3). b, Under control conditions, one neuron (the lower, left) shows a high spiking activity, as indicated by the activity pattern (red circles in the grid windows). After acute treatment with an inhibitor cocktail (TTX, CNQX, APV), spiking behavior is blocked (yellow squares; traces in c), non-spike-like activity events become visible on the soma and in the periphery. Shape and number of residual activity signals are quite diverse (bright blue squares; traces in c). c; Grid window-specific signal traces with the corresponding activity marks (red) as indicated in b. d, Activity hotspot in the periphery, in a varicosity-like structure. The activity hotspot is indicated by a contrast-enhanced average intensity projection. Image 496 shows the low activity state, while image 2124 indicates the high activity state. The corresponding grid window is indicated in (b).