An open source tool for automatic spatiotemporal assessment of calcium transients and local ‘signal-close-to-noise’ activity in calcium imaging data
Fig 4
Calcium spike-detection under stringent computing conditions.
a, Confocal imaging of synchronously spiking glia-derived neurons (re-computing of earlier data; [40]. Cells were loaded with calcium indicator OGB1 to label glial cells and neurons. Spontaneous activity was induced by inhibition of GABAergic signaling with bicuculline to induce the spiking behavior in the neural network. A magenta arrow labels all neurons. Computed loci are shown. The average signal trace representing all computed loci is shown. b, Imaging was performed under low-light conditions. The raw image represents this imaging situation. Loci of computed activity events are shown on a brightness-contrast corrected image of the neurons. Under control conditions, spontaneous spiking is observed on the somata, but also in the periphery of the neurons. Spike blockade (TTX, CNQX) correlates with a reduced number of computed activity events. Graph: Raw intensity values are plotted against the frame number. The calcium spikes are efficiently blocked by TTX and CNQX. Scale bar in a: 100μm; in b: 50 μm.