Systems-level computational modeling demonstrates fuel selection switching in high capacity running and low capacity running rats
Fig 3
HCR and LCR metabolic constraint-based solutions.
(A) HCR constraint-based solution: Eq 1 (in Methods) was solved at each time point for internal reaction fluxes using given carbohydrate, FA, O2, and CO2 transport fluxes. Transport fluxes were derived from HCR O2 and CO2 flux data [30] shown in Fig 2. (Inset) Zoomed in view of HCR mitochondrial FA transport and beta-oxidation enzyme fluxes. (B) LCR constraint-based solution: Eq 1 (in Methods) was solved at each time point for internal reaction fluxes using given carbohydrate, FA, O2, and CO2 fluxes [30] shown in Fig 2. (Inset) Zoomed in view of LCR mitochondrial FA transport and beta-oxidation enzyme fluxes. (C) The ATPase (ATP → ADP + Pi) flux (circles) from the HCR constraint-based solution in panel A was used to estimate the ATPase rate with time for subsequent simulations.