Identification of allosteric inhibitors of the ecto-5'-nucleotidase (CD73) targeting the dimer interface
Fig 5
Determination of the kinetics inhibition profiles for the most representative compounds.
Secondary plots and double-reciprocal of steady state rate constants as a function of AMP concentration in the absence (circles) or with increasing concentrations of hit compounds (squares, triangles and stars). (A): RR3 at 0, 0.4, 0.8 and 1.6 μM; (B): RR4 at 0, 0.3, 0.6 and 1.2 μM; (C): RR6 at 0, 0.25, 0.5 and 1.0 μM; (D): RR20 at 0, 0.3, 0.6 and 1.2 μM.