Mechanical feedback coordinates cell wall expansion and assembly in yeast mating morphogenesis
Fig 5
Control of mating projection size.
(A) Diagram of a growing mating projection showing the mating projection radius R and length scale of the secretion region (green), λX. (B) Theoretically predicted dependence of the projection radius R with the length scale of the secretion region, λX, and the strength of mechanical feedback, Γ. (C-D) Confocal images of WT (C) and spa2Δ (D) mutant cells growing mating projections. The cell wall is labeled with calcofluor (white) and the exocytosis profile is defined by Sec3-GFP (green). Scale bar, 1 μm. (E) Measured average cell radius, R, and exocytosis length scale, λX, for mid2Δ and wsc1Δ mutants in both WT and spa2Δ backgrounds (mid2Δ, N = 6; wsc1Δ, N = 9; spa2Δ mid2Δ, N = 7; spa2Δ wsc1Δ, N = 6), as well as for WT (N = 7) and spa2Δ (N = 6) cells. Mean and standard deviation are shown.