Scalable multi-sample single-cell data analysis by Partition-Assisted Clustering and Multiple Alignments of Networks
Fig 4
t-SNE visualization of clustering methods.
We compare the clustering results between hand-gate, (Lloyd’s) kmeans, SPADE, flowMeans, bPAC, and dPAC labels. Each t-SNE plot contains all gated cell events from the hand-gated CyTOF data with different set of colored labels. The colored labels denote different subpopulations within each plot; however, the colors do not have cross-plot meaning. The subpopulation numbers for all methods were set to be the same as that of hand-gated results (24 subpopulations). PAC methods achieve a significantly better convergence to the hand-gate labels than alternative methods.