Genetic drift and selection in many-allele range expansions
Fig 10
A four-color E. coli range expansion (left) and the superimposed binary masks of each channel (right) [34].
Images were acquired for four overlapping quadrants and stitched together to obtain a single image with a large field of view. Overlapping regions were blended to minimize inhomogeneities. To obtain the binary masks, pixels with fluorescence above background noise were marked as “on.” A visual comparison of the raw data and the masks confirm that our binary masks accurately reflect the location and shape of individual sectors.