Strawberry: Fast and accurate genome-guided transcript reconstruction and quantification from RNA-Seq
Fig 6
Read alignments and reconstructed transcripts at gene NAT14 using HepG2 data.
A new isoform, transcript.14285.3 (shown as the middle one), has been identified by Strawberry. The junction reads that support the new AS event (alternative 3 splice site) are highlighted. The two ends of a read-pair are in the same color. A total 7 uniquely mapped read-pairs supports the novel junction. This figure is made by IGV (http://software.broadinstitute.org/software/igv/).