Concerted regulation of npc2 binding to endosomal/lysosomal membranes by bis(monoacylglycero)phosphate and sphingomyelin
Fig 6
Schematic representation of the membrane binding of npc2.
The thumbnail images for npc2apo (green) and npc2chol-bound (red) are placed on the y-axis based on the pmf data (Fig 3A) of the bmp:chol:sm:dopg:popc = 20:10:0:0:70 (System 5) (low chol), bmp:chol:sm:dopg:popc = 20:35:0:0:45 (System 6) (high chol), and bmp:chol:sm:dopg:popc = 20:10:20:0:50 (System 7) (low chol with sm) systems. A putative cycle for transferring cholesterol from high chol to low chol membranes are indicated by thick arrows. The competing processes (the interconversion between the two binding modes and binding to sm-containing membranes) are indicated by dashed arrows.