Concerted regulation of npc2 binding to endosomal/lysosomal membranes by bis(monoacylglycero)phosphate and sphingomyelin
Fig 5
Cholesterol-binding free energy change upon membrane binding.
A) Thermodynamic cycle for the estimation of cholesterol binding free energy change. Solid arrows represent the transformations performed in this work and dashed arrows represent the alternative transformations. B) The cholesterol binding free energy change as a function of min zr, calculated by ΔΔGchol-b.(min zr) = ΔGchol-b.(min zr) − ΔGapo(min zr). The labels indicate membrane content in molar fractions for a mixture of bmp:chol:sm:dopg:popc, and the corresponding system numbers (Table 1) are provided in parentheses.