Competitive tuning: Competition's role in setting the frequency-dependence of Ca2+-dependent proteins
Fig 5
Competitive tuning explains intermolecular crosstalk.
(A) Simulations of CaMKII phosphorylation in our isolated model with and without inclusion of Ng. (B) Simulations of CaMKII phosphorylation in our competitive model with and without Ng. (C) CaMKII activity in WT and Ng-/- knockout mice from Krucker et al. Simulations were performed to replicate the experimental method of Krucker et al. as closely as possible. (D) The average bound concentration (Cb) of each CaM binding protein in semi-isolated models as a function of Ng concentration. AC8-Ct and AC8-Nt exhibit the greatest relative change in CaM-binding (Cb, Eq 1) as Ng concentration decreases. (E) The average bound concentration (Cb) of each CaM binding protein in the competitive model as a function of Ng concentration. For a decreasing Ng concentration, AC8-Ct and AC8-Nt again exhibit the greatest relative change in CaM-binding. (F) Comparing the semi-isolated (dotted traces) to the competitive (solid traces) model shows that only in the competitive model does summed AC8 (AC8-Nt + AC8-Ct, dark red) mirror the loss in CaM-CaMKII binding as Ng concentration decreases.