The role of glutamate in neuronal ion homeostasis: A case study of spreading depolarization
Fig 8
Inhibitor of astrocytic glutamate transporters, TFB-TBOA, significantly increases the duration of SD.
Here we show the network and single cell properties of SD in control and TFB-TBOA treated groups. Representative time traces of membrane potential of individual pyramidal neurons (bottom trace) and network level (top trace) in layers 2-3 of visual cortex recorded using whole-cell patch clamp and extracellular recording techniques respectively in control (n = 9) and TFB-TBOA treated groups (n = 9) are shown in panels (a–c). (d-e) SD duration defined as the time from the initiation of rapid depolarization in individual neurons and network to the time when the membrane potential repolarizes to its pre-SD value. TFB-TBOA prolonged the duration of SD both at the network level (d) (n = 9, p<0.001) and single cell level (e) (n = 9, p<0.01).