Image-based model of the spectrin cytoskeleton for red blood cell simulation
Fig 3
Cryoelectron tomography and pattern matching of RBC skeletons.
(a) A virtual slice of a cryoelectron tomogram of a red blood cell skeleton. Putative junctional complexes containing the actin protofilaments are indicated with arrows. Structures inside dashed lines are uncharacterized protein clusters (circles) and lipidic remains (squares), (b) Cross-correlation map resulting from comparing the actin template with the tomogram. The brighter the spot, the higher the probability of a good match, (c) The corresponding cross-correlation coefficient plot with a line indicating the cutoff value used. The 2nd knee of the curve is used to avoid false negatives, (d) The identified actin protofilaments overlaid on the actual densities of the tomogram with obvious false positives discarded (for example, some spots with high cross-correlation values are found at locations of lipid remains and unknown protein clusters).