Polymodal allosteric regulation of Type 1 Serine/Threonine Kinase Receptors via a conserved electrostatic lock
Fig 6
BRE-luciferase reporter assay for different STKR1 receptor mutants.
(a) C2C12/BRE-luc cells were either untreated (grey bars), treated with 1μM FK506 (red bars) or 50 ng/ml BMP6 (blue bars) overnight, followed by a dual-Luciferase reporter assay. Renilla luciferase reporter pRL-TK plasmid was used as an internal control. (b) The relative luciferase unit difference between FK506 treatment and untreatment for the STKR1 receptor mutants. Data are presented as the mean ± S.E.M. (standard error), each performed in triplicate. ns., no significant difference; *p< 0.05, **p< 0.01, ***p< 0.001.