Reconstructing the regulatory circuit of cell fate determination in yeast mating response
Fig 3
Mechanism of PSDA and identified regulation events in shmooing cells.
(A) PSDA deconvolutes the gene-specific regulation by modeling the effect of the physiological response of the yeast cells. (B) Examples for PSDA results of six different strains. The dashed line indicates the protein dynamic changes caused by global regulation and the dots represent the observed protein abundance. Time window of gene-specific regulation are gray shaded. Fold change of regulation is indicated; green arrow (activation), red arrow (inhibition). (C) Identified gene regulation events in shmooing cells. Values are the log-transformed fold change of the regulation level. Distinct temporal modes were distinguished by k-means clustering, and the 50% activation/inhibition time for each cluster is represented by the dashed line. C1-C6 denote clusters 1–6. Right column, proteins responsible for the cell cycle (dark brown), conjunction (brown) and chemical response (light brown).