Automatic analysis and 3D-modelling of Hi-C data using TADbit reveals structural features of the fly chromatin colors
Fig 2
Hi-C interaction maps at 100 kb resolution for the entire Drosophila genome.
(a) Raw, filtered and normalized genome-wide interaction maps for the BR dataset. Only after the normalization of the data, the enriched interaction between centromere regions of the Drosophila chromosomes can be observed. (b) Normalized maps for the TR1 and TR2 datasets. (c) Comparison of the normalized Hi-C maps between the three datasets at 100 kb resolution. The Spearman correlation was computed between off-diagonal regions as a function of their genomic distance. (d) Matrices of Pearson correlation coefficients of main eigenvectors from the three Hi-C datasets (that is, BR, TR1 and TR2). The data shows the expected high correlation of the top three eigenvectors [32]. (e) Genomic coverage of the mapped reads per chromosome from the SUM dataset. (f) Hi-C normalized interaction matrix at 100 kb resolution for the SUM dataset. The three main eigenvectors of the normalized interaction matrix mark the position of centromeres (E1), chromosomes (E2), and chromosome arms (E3). TADbit automatically generated all the plots in the figure.