Identifying direct contacts between protein complex subunits from their conditional dependence in proteomics datasets
Fig 8
Identification of direct contacts between C14ORF142 and the human EKC/KEOPS tRNA modification complex.
A. Highly conserved across both prokaryotes and eukaryotes, the EKC/KEOPS complex is thought to consist of 5 proteins, visualized here for the yeast complex based on a combination of modeling and crystal structures (PDB entries 4WX8, 5JMV, 3EN9 and 4WW5). B. Conditionally dependent interactions among subunits of the human protein complex highlight specific contacts that match closely to those observed between the yeast proteins, and additionally implicate the human protein C14ORF142 as directly bound to OSGEP and LAGE3. C. Using the yeast structure as a homology template, a 3D model was built of the 4 known human subunits and C14ORF142, taking advantage of C14ORF142’s distant similarity to the yeast Gon7 subunit.