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MFPred: Rapid and accurate prediction of protein-peptide recognition multispecificity using self-consistent mean field theory

Fig 5

Proof-of-concept for design.

Changes in specificity profile upon granzyme B protease mutation are recapitulated by MFPred. (a) Experimental (bold) specificity (average of Harris et al. [46] and Ruggles et al. [47]) and predicted P3 specificity for WT granzyme B protease. (b)-(c), WT granzyme B protease structure. (d) R192E granzyme B protease active site. (e) Experimental specificity (bold) [46] and predicted P3 specificity for R192E granzyme B protease. (f) R192E/N218A granzyme B protease active site. (g) Experimental specificity (bold) [47] and predicted P3 specificity for R192E/N218A granzyme B protease.

Fig 5

doi: https://doi.org/10.1371/journal.pcbi.1005614.g005