Systematic, network-based characterization of therapeutic target inhibitors
Fig 3
Validation of predicted novel modulators for MYC and STAT3.
(A) Effect of computationally predicted drug modulators on MYC activity in luciferase assay. The compounds were tested by MYC luciferase reporter assay in MCF7 cell line. Concentrations for each compound are shown in S4 Table. The reporter activity for each compound was normalized to cell titer and compared to DMSO control. We compared drug induced activity vs. DMSO control using t-test. (p< 0.01: ***; p < 0.05: **; p<0.1: *) (B) STAT3 luciferase reporter assay results in MCF7 (blue) and SNB19 (red). The compounds were tested by STAT3 luciferase reporter assay in MCF7 and SNB19 cell line. Each compound was tested at 10uM. The activity for each drug is computed using the reporter activity normalized to cell titer and compared to DMSO control. We compared drug induced activity vs. DMSO control using t-test. (p< 0.01: ***; p < 0.05: **; p<0.1: *).