Mitochondrial respiration and ROS emission during β-oxidation in the heart: An experimental-computational study
Fig 5
Modeling studies of mitochondrial respiration, H2O2 emission and ROS scavenging intermediates as a function of PCoA concentration and proton conductance.
The steady state behavior of VO2 in the model was analyzed for the same range of PCoA as in Fig 3 mimicking those used in the experiments under states 4 (A) and 3 (C) respiration (blue traces) shown in Fig 2A and 2B. Also depicted in black traces are the rates of H2O2 emission corresponding to the same simulations. Panels B and D display the steady state concentration of main components of matrix antioxidant systems (GSH, Trx(SH)2, NADPH) under both states 4 (B) and 3 (D) respiration. The parameterization and initial conditions employed in the simulations are described in S1 Text together with the Matlab code for the full computational model.