Post-transcriptional regulation across human tissues
Fig 3
Concerted variability in the relative protein-to-RNA (rPTR) ratio of functional gene-sets across tissue-types.
(a) mRNAs coding for the ribosomal proteins, NADH dehydrogenase and respiratory proteins have higher protein-to-mRNA ratios in kidney as compared to the median across the other 11 tissues (FDR < 1%). In contrast mRNAs genes functioning in Rac GTPase binding have lower protein-to-mRNA ratios (FDR < 1%). (b) The stomach also shows significant rPTR variation, with low rPTR for the ribosomal proteins and high rPTR for tRNA-aminoacylation (FDR < 1%). (c) Summary of rPTR variability, as depicted in panel (a-b), across all tissues and many gene ontology (GO) terms. Metabolic pathways and functional gene-sets that show statistically significant (FDR < 1%) variability in the relative protein-to-mRNA ratios across the 12 tissue types. All data are displayed on a log10 scale, and functionally related gene-sets are marked with the same color. (d) The reproducibility of rPTR estimates across estimates from different studies is estimated as the correlation between the median rPTRs for GO terms showing significant enrichment as shown in panels (a-c). See Methods, S2 and S3 Figs.